Multi-Page PapersVolume 10, Spring 2016

Characterizing Intracellular Localization and Chromatin Remodeling Role of CHD6 in Human Fetal Development and in Glioblastoma

Noah Basri1,

Nadejda Tsankova1

1 Icahn School of Medicine at Mount Sinai, Departments of Pathology and Neuroscience, Friedman Brain Institute

Abstract

CHD6, a member of the CHD family of DNA-binding, chromatin-modifying proteins, has been shown to be upregulated in glioblastoma. Using immunofluorescence the intracellular region to which CHD6 localizes can be determined. Through the use of chromatin Immunoprecipitation (ChIP), candidate genomic loci enriched for CHD6 can be determined. Immunofluorescence and ChIP results for CHD6 using a current protocol are presented. Notably, immunofluorescence of fetal germinal matrix and adult subventricular zone showed principally cytoplasmic localization of CHD6, contrary to expectation. CHD6 ChIP showed partial enrichment in certain genes of possible significance to glioblastoma in fetal tissue relative to the positive control. A higher sample size is needed to test and evaluate the significance of these findings. The results presented here reveal new insights into the changes in the epigenetic landscape contributing to glioblastoma and the manipulation of CHD6 expression could be considered for treating specific incidents of glioblastoma.